Human mesenchymal stem/stromal cells, or MSCs, are key constituents of future therapeutics, engineered tissues and medical devices and are presently in use in more than 400 clinical trials. Bone marrow-derived MSC (BM-MSC) have traditionally been the most extensively used MSC, but MSC can be isolated from numerous tissues of the body including fat, umbilical cord blood, dental pulp, Wharton’s jelly and peripheral blood. In recent years, human Adipose (or fat) tissue-derived MSC (AD-MSC) are progressively used in studies because of adipose tissue having a greater frequency of MSC than bone marrow and the comparative effortlessness of collection.

Autologous stem cell therapy in the arena of regenerative medicine encompasses harvesting, isolating the stem and regenerative cells from adipose tissue, bone marrow and /or peripheral blood and directing the cells back to the patient. Effectual, authenticated compliant office- based technique for isolating adipose, blood and bone marrow derived stem cells.

Adipose-derived stem cells (ASCs) have the same multipotency as bone marrow mesenchymal stem cells. Thus, adipose tissue substitutes for bone marrow as a foundation of stem cells. Preferably, a technique to isolate and distinguish ASCs should be established and adipose tissue should be used as a source of stem cells for regenerative medicine. Manual and automatic stem cell separation techniques are employed to detach ASCs from adipose tissue. At StemCellCareIndia, we describe the potential of adipose-derived stem cells for regeneration medicine, clinical application and practicality of fat grafting. Fat tissue offers the major volume of adult stem cells, while bone marrow offers growth factors to help with the repair process.