Skin is the biggest yet delicate organ of our body, encompassing of chiefly three distinguished layers, namely, outer epidermis, middle dermis and the inmost hypodermis; each contributing distinctively towards maintaining structural and functional integrity of the organ. Skin has some different purposeful characteristics; it serves as a protective covering, saving internal vital organs from physical, chemical and environmental attacks. Existence of nerve endings in the skin offers sensation, while melanin gives color to the skin.
It serves as one of the self-renewing organs, with a sturdy potential to repair and heal small cuts, scratches on its own; because of the presence of resident adult stem cells. However, their number is not adequate enough to take charge over major injuries like deep cuts or abrasions, skin burns and ulcers. These skin injuries/wounds can cause severe mutilation to the deepest layer of the skin; therefore, might compromise normal function of the skin.
Skin injuries/wounds, if overlooked for a longer period of time can cause severe infection, resulting in the life-threatening complications, such as gangrene, sepsis, cellulites, etc. Reparation of skin injuries can be possible in three steps, i.e. inflammation, proliferation and remodeling; and numerous skin cells are involved in the procedure of healing, such as keratinocytes, fibroblasts, vascular endothelial cells, etc. But in circumstances of deeper injuries or wounds, the skin repair necessitates instantaneous medical aid. Some of the common groupings of injuries include:
- Deep Cuts
- Gastric ulcers
- Diabetic Foot Ulcers
Conventionally, the only available treatment option for the aforementioned skin issues was skin grafts, attained from allogeneic as well as autologous sources. However, the applications of conventionally available options have often been found to be related with number of limitations, such as scarcity of autografts, particularly for patients with huge surface area of burns, skin infections in case of allografts, aesthetically unattractive finishing, etc.
For that matter, majority of scientific community has been forced to ponder the new effectually simple approach towards wound healing. Accordingly, a combinational methodology of adipose tissue derived stem cells activated with their own platelet rich plasma has been proven to be effective as a prospective wound healing strategy. A number of studies have been implemented to support the scientific advancements and have confirmed amplified healing rates and improved functional attributes by promoting quicker proliferation of lost or degenerated cells. So far so good, no hostile effects have been faced in any of the ongoing clinical study; nor have we stumbled across any during our treatments!
The treatment at our clinic is concentrated predominantly on improving the patient’s quality of life Stem cell processing method
Umbilical cord collection and preliminary testings
It is a common practice in India for mommies to voluntarily donate the umbilical cord blood from the birth of their fit full-term kids. Every mom who enters a hospital in India to give birth is spontaneously tested for all main sicknesses. Comprehensive family histories are also taken from each mother before donation. Only females who have been fully screened and found to be totally healthy are allowable to donate their umbilical cord and umbilical cord blood.
Second round of testing
The second step in the screening procedure happens in laboratory once the collected samples have been verified negatively for communicable ailments; it is then transported to our laboratories and officially becomes the property. From this phase, it is mandatory for each and every sample to be processed and provided to patients. The gathered sample starts a second round of testing and while following donor’s prerequisite also carries out testing for cytomegalovirus, micro-organisms (aerobic bacteria, anaerobic bacteria, fungi, etc.), survival rate, stem cell surface indicators and stem cell biological characteristics (colony forming aptitude, differential capability) in each step to safeguard the highest quality to its cell products.
The stem cells derived from each umbilical cord are sowed into a flask filled with culture medium. This medium does not have any animal products (such as fetal calf serum) but it is heightened with cell growth factors. The flasks are positioned in a sterilized, temperature and humidity controlled incubator. The stem cells are lengthened in the culture medium. Once the culturing is done, the culture mediums are splashed away and are harvested.
The cell products are then cryo-preserved and stored at a consistent -196 degree Celsius temperature with automatic supplement of liquid nitrogen using the exceedingly advanced Thermogenesis BioArchive system. This technology permits for integrated control rate of freezing that absolutely avoids unexpected drops in temperature. Our cryo-preservation storage equipment is also fortified with 24h real-time control system and in any situation of power-failure or other emergency; a redundant fail-safe power supply guarantees that normal operation is implemented. Our vacuum protection system can uphold appropriate temperature for 20 days without supplement of liquid nitrogen.
Packing and transport
All consignments of stem cells undergo final testing. During this procedure, they are also tested for quantity and quality. Once the stem cells are reckoned safe, each unit is positioned into either a sterile vial or a sterile IV bag for future transplantation. All merchandises are separately cataloged and traced while in transit. Our cell transplants come to pass two to three days a week. All of our cells are processed on an as-needed basis. All of our cell products are treated and kept fresh, giving us the highest stem cell sustainability count and the maximum effectiveness.
Stem cell procedure
The doctors in our affiliated hospitals use several injection procedures to deliver stem cells. Contingent on each patient’s particular condition, one or multiple approaches of delivery will be used during a standard treatment sitting so as to maximize safety and effectiveness. It is imperative to remember that Stem Cell Care India protocols always highlight safety first and the injections approaches provided are slightly invasive but still a very concentrated on delivering the stem cells as close as possible from the injury location.
1. What is PRP?
PRP is the platelet Rich Plasma. Plasma is the liquid element of the blood, which serves as the host for all the cellular constituents of the blood like red blood cells, white blood cells and platelets. Thus platelets naturally make up about 10% of the blood’s cellular element. With the assistance of a technology, it is possible to flip the ratio of platelets upto 90% concentration. Consequently, it is named as the Platelet Rich Plasma.
2. What is PRP treatment and how does it work?
Platelets are acknowledged as the first respondents after the injury. Instantaneously after the injury, the human body begins provoking an immune response permitting platelets and other immune constituents to migrate at the location of injury. At the targeted location, these platelets are acknowledged to secrete numerous growth factors and clotting factors to initiate the healing process, encourage blood vessels formations as well as collagen synthesis. These platelets are also acknowledged to trigger the secretion of several bioactive molecules, which can attract many macrophages, mesenchymal stem cells and osteoblasts. PRP is a non-surgical healing treatment used in the arena of regenerative medicines to stimulate and augment healing. In a simple language, PRP is considered as the mega doses prepared from the components of our own body for our own healing.
3. What varieties of conditions can be treated with PRP?
As per the current research, all the orthopaedic related complications such as soft tissue injuries, tendonitis, ligament sprains and muscle tears can be treated well with PRP treatment. It is as well found to be very advantageous in anti-aging treatment, hair restoration and wound healing such as diabetes foot.
4. Is PRP treatment painful?
Patient normally tolerates the treatment well both with and without anesthesia. However, post injection inflammation in expected given the PRP induced inflammatory response. But this discomposure such as inflammation are perceived in the typical injected region which will not be for more than 48 hrs.
5. How long does it take the PRP to work?
Many of the individuals have noticed some or the other type of improvement contingent upon the problem by 2-3 weeks after the PRP treatment. Symptom improvement has been detected to be slow and subtle with the passage of time, with usual grumble of original pain being replaced by the discomfort. Augmented endurance and strength are typical observation instantaneously after the treatment.
6. What is involved in the preparation of PRP?
A trivial quantity of blood is withdrawn usually 30 ml from the peripheral vein of the patient. The blood is then retained in the centrifuge, to isolate platelet from rest of the constituents. On the therapeutic facets, platelet count is supplemented 5 fold with 10-15 fold reduction in the volume after the processing. The whole process takes not more than 20 mins to upsurge the concentration of platelet accompanied by the innate stimulation of stem cells for speedy proliferation, differentiation and regeneration. The concentrated PRP is then vaccinated around the area or in the joints to accelerate the process of healing.