Step1: Umbilical Cord Collection and Initial Testings
It is a common practice in China for moms to willingly donate the umbilical cord blood from the birth of their healthy full-term children. Every mommy who enters a hospital in China to give birth is automatically substantiated for all major infections. Detailed family histories are also taken from each mother before donation. StemCellCareIndia follows the People’s Republic of China strict regulation about blood donation and not less than five test rounds are being conducted by nationally qualified third-parties on potential donors for pathogen detection. Only women who have been fully screened and found to be totally healthy are permitted to donate their umbilical cord and umbilical cord blood.
Step 2: Second Round of Testing
The second step in the screening process happens in laboratory once the gathered samples have been verified negatively for communicable sicknesses; it is then moved to our laboratories and officially becomes the property of ours. From this segment, we are accountable for each and every sample that is processed and offered to patients. The gathered sample starts a second round of testing and while following donor’s required from the USFDA and AABB, we also perform the investigation for cytomegalo virus, exogenous virus (human T-cell leukemia virus, HTLV), micro-organisms (aerobic bacteria, anaerobic bacteria, fungi, etc.), survival rate, stem cell surface indicators and stem cell biological characteristics (colony forming aptitude, differential capacity) in each step to promise the highest quality to its cell products.
Step 3: Separation
Obtaining of the cord blood serum and preparing the stem cells for culturing is accomplished by splitting the desired constituents from the cord blood. The blood cells need to be removed from the blood serum and stem cells so as to not obstruct with future injections. A centrifuge is used to separate the serum and stem cells from the red cells and platelets. This is particularly refined and specialized separation process. After separation has been completed, the stem cells are removed for culture.
Step 4: Culturing
The stem cells derived from each umbilical cord are spread into a flask filled with culture medium. This medium does not incorporate any animal products (such as fetal calf serum) but it is enhanced with cell growth factors. The flasks are sited in a sterilized, temperature and humidity controlled incubator. The stem cells are protracted in the culture medium. Once the culturing is complete, the culture medium is washed away and is collected.
Step 5: Storage
The cell products are then cryo-preserved and stored at a consistent -196 degree Celsius temperature with automatic supplement of liquid nitrogen using the significantly advanced Thermogenesis Bio Archive system. This technology offers integrated control rate of freezing that totally sidesteps unanticipated drops in temperature. Our cryo-preservation storage equipment is also equipped with 24h real-time control system and in any case of power-failure or other emergency, a redundant goof proof power supply ensures that normal operation is carried out. Our vacuum protection system can retain appropriate temperature for 20 days without supplement of liquid nitrogen.
Step 6: Packing and transport
All lots of stem cells go through final testing. During this process, they are also tested for quantity and quality. Once the stem cells are expected to be safe, each one unit is placed into either a sterile vial or a sterile IV bag for future transplantation. All products are individually characterized and tracked while in shipment. Our cell transplants come about two to three days a week. All of our cells are processed on an as-needed basis. All of our cell products are processed and kept fresh, giving us the topmost stem cell feasibility count and the maximum efficacy.